Synthesis of poly(meth)acrylates with thioether and tertiary sulfonium groups by ARGET ATRP and their use as siRNA delivery agents.
Identifieur interne : 000295 ( Main/Exploration ); précédent : 000294; suivant : 000296Synthesis of poly(meth)acrylates with thioether and tertiary sulfonium groups by ARGET ATRP and their use as siRNA delivery agents.
Auteurs : Matthew C. Mackenzie [États-Unis] ; Arun R. Shrivats ; Dominik Konkolewicz ; Saadyah E. Averick ; Michael C. Mcdermott ; Jeffrey O. Hollinger ; Krzysztof MatyjaszewskiSource :
- Biomacromolecules [ 1526-4602 ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Composés de sulfonium (), Crâne (cytologie), Glyceraldehyde 3-phosphate dehydrogenases (génétique), Lignée cellulaire, Matériaux biocompatibles (synthèse chimique), Microscopie à force atomique (), Microscopie électronique à balayage (), Ostéoblastes (cytologie), Ostéoblastes (métabolisme), Petit ARN interférent (administration et posologie), Petit ARN interférent (génétique), Poly(acides méthacryliques) (), Poly(acides méthacryliques) (synthèse chimique), Polyéthylène glycols, Souris.
- MESH :
- administration et posologie : Petit ARN interférent.
- cytologie : Crâne, Ostéoblastes.
- génétique : Glyceraldehyde 3-phosphate dehydrogenases, Petit ARN interférent.
- métabolisme : Ostéoblastes.
- synthèse chimique : Matériaux biocompatibles, Poly(acides méthacryliques).
- Animaux, Composés de sulfonium, Lignée cellulaire, Microscopie à force atomique, Microscopie électronique à balayage, Poly(acides méthacryliques), Polyéthylène glycols, Souris.
English descriptors
- KwdEn :
- Animals, Biocompatible Materials (chemical synthesis), Cell Line, Glyceraldehyde-3-Phosphate Dehydrogenases (genetics), Mice, Microscopy, Atomic Force (methods), Microscopy, Electron, Scanning (methods), Osteoblasts (cytology), Osteoblasts (metabolism), Polyethylene Glycols, Polymethacrylic Acids (chemical synthesis), Polymethacrylic Acids (chemistry), RNA, Small Interfering (administration & dosage), RNA, Small Interfering (genetics), Skull (cytology), Sulfonium Compounds (chemistry).
- MESH :
- chemical , administration & dosage : RNA, Small Interfering.
- chemical , chemical synthesis : Biocompatible Materials, Polymethacrylic Acids.
- chemical , chemistry : Polymethacrylic Acids, Sulfonium Compounds.
- chemical , genetics : Glyceraldehyde-3-Phosphate Dehydrogenases, RNA, Small Interfering.
- cytology : Osteoblasts, Skull.
- metabolism : Osteoblasts.
- methods : Microscopy, Atomic Force, Microscopy, Electron, Scanning.
- Animals, Cell Line, Mice, Polyethylene Glycols.
Abstract
The field of RNA interference depends on the development of safe and efficient carriers for short interfering ribonucleic acid (siRNA) delivery. Conventional cationic monomers for siRNA delivery have utilized the nitrogen heteroatom to produce cationic charges. Here, we polymerized cationic sulfonium (meth)acrylate by activators regenerated by electron transfer (ARGET) atom transfer radical polymerization (ATRP) to form polymers with narrow molecular weight distributions for siRNA delivery. The tertiary sulfonium species was stable toward dealkylation in water but less stable in the polar aprotic solvent dimethyl sulfoxide. Block copolymers poly(ethylene oxide) with poly(meth)acrylate containing sulfonium moieties were prepared as an siRNA delivery platform. Results suggested block copolymers were biocompatible up to 50 μg/mL in vitro and formed polyplexes with siRNA. Additionally, block copolymers protected siRNAs against endonuclease digestion and facilitated knockdown of glyceraldehyde 3-phosphate dehydrogenase (Gapdh) mRNA expression in murine calvarial preosteoblasts. The versatility, biocompatibility, and cationic nature of these tertiary sulfonium groups are expected to find widespread biological applications.
DOI: 10.1021/bm501449a
PubMed: 25515324
Affiliations:
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Averick</name></author><author><name sortKey="Mcdermott, Michael C" sort="Mcdermott, Michael C" uniqKey="Mcdermott M" first="Michael C" last="Mcdermott">Michael C. Mcdermott</name></author><author><name sortKey="Hollinger, Jeffrey O" sort="Hollinger, Jeffrey O" uniqKey="Hollinger J" first="Jeffrey O" last="Hollinger">Jeffrey O. Hollinger</name></author><author><name sortKey="Matyjaszewski, Krzysztof" sort="Matyjaszewski, Krzysztof" uniqKey="Matyjaszewski K" first="Krzysztof" last="Matyjaszewski">Krzysztof Matyjaszewski</name></author></analytic><series><title level="j">Biomacromolecules</title><idno type="eISSN">1526-4602</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Biocompatible Materials (chemical synthesis)</term><term>Cell Line</term><term>Glyceraldehyde-3-Phosphate Dehydrogenases (genetics)</term><term>Mice</term><term>Microscopy, Atomic Force (methods)</term><term>Microscopy, Electron, Scanning (methods)</term><term>Osteoblasts (cytology)</term><term>Osteoblasts (metabolism)</term><term>Polyethylene Glycols</term><term>Polymethacrylic Acids (chemical synthesis)</term><term>Polymethacrylic Acids (chemistry)</term><term>RNA, Small Interfering (administration & dosage)</term><term>RNA, Small Interfering (genetics)</term><term>Skull (cytology)</term><term>Sulfonium Compounds (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Composés de sulfonium ()</term><term>Crâne (cytologie)</term><term>Glyceraldehyde 3-phosphate dehydrogenases (génétique)</term><term>Lignée cellulaire</term><term>Matériaux biocompatibles (synthèse chimique)</term><term>Microscopie à force atomique ()</term><term>Microscopie électronique à balayage ()</term><term>Ostéoblastes (cytologie)</term><term>Ostéoblastes (métabolisme)</term><term>Petit ARN interférent (administration et posologie)</term><term>Petit ARN interférent (génétique)</term><term>Poly(acides méthacryliques) ()</term><term>Poly(acides méthacryliques) (synthèse chimique)</term><term>Polyéthylène glycols</term><term>Souris</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>RNA, Small Interfering</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Biocompatible Materials</term><term>Polymethacrylic Acids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Polymethacrylic Acids</term><term>Sulfonium Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glyceraldehyde-3-Phosphate Dehydrogenases</term><term>RNA, Small Interfering</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Petit ARN interférent</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Crâne</term><term>Ostéoblastes</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Osteoblasts</term><term>Skull</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glyceraldehyde 3-phosphate dehydrogenases</term><term>Petit ARN interférent</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Osteoblasts</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microscopy, Atomic Force</term><term>Microscopy, Electron, Scanning</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Ostéoblastes</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Matériaux biocompatibles</term><term>Poly(acides méthacryliques)</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Mice</term><term>Polyethylene Glycols</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Composés de sulfonium</term><term>Lignée cellulaire</term><term>Microscopie à force atomique</term><term>Microscopie électronique à balayage</term><term>Poly(acides méthacryliques)</term><term>Polyéthylène glycols</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The field of RNA interference depends on the development of safe and efficient carriers for short interfering ribonucleic acid (siRNA) delivery. Conventional cationic monomers for siRNA delivery have utilized the nitrogen heteroatom to produce cationic charges. Here, we polymerized cationic sulfonium (meth)acrylate by activators regenerated by electron transfer (ARGET) atom transfer radical polymerization (ATRP) to form polymers with narrow molecular weight distributions for siRNA delivery. The tertiary sulfonium species was stable toward dealkylation in water but less stable in the polar aprotic solvent dimethyl sulfoxide. Block copolymers poly(ethylene oxide) with poly(meth)acrylate containing sulfonium moieties were prepared as an siRNA delivery platform. Results suggested block copolymers were biocompatible up to 50 μg/mL in vitro and formed polyplexes with siRNA. Additionally, block copolymers protected siRNAs against endonuclease digestion and facilitated knockdown of glyceraldehyde 3-phosphate dehydrogenase (Gapdh) mRNA expression in murine calvarial preosteoblasts. The versatility, biocompatibility, and cationic nature of these tertiary sulfonium groups are expected to find widespread biological applications.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Averick, Saadyah E" sort="Averick, Saadyah E" uniqKey="Averick S" first="Saadyah E" last="Averick">Saadyah E. Averick</name><name sortKey="Hollinger, Jeffrey O" sort="Hollinger, Jeffrey O" uniqKey="Hollinger J" first="Jeffrey O" last="Hollinger">Jeffrey O. Hollinger</name><name sortKey="Konkolewicz, Dominik" sort="Konkolewicz, Dominik" uniqKey="Konkolewicz D" first="Dominik" last="Konkolewicz">Dominik Konkolewicz</name><name sortKey="Matyjaszewski, Krzysztof" sort="Matyjaszewski, Krzysztof" uniqKey="Matyjaszewski K" first="Krzysztof" last="Matyjaszewski">Krzysztof Matyjaszewski</name><name sortKey="Mcdermott, Michael C" sort="Mcdermott, Michael C" uniqKey="Mcdermott M" first="Michael C" last="Mcdermott">Michael C. Mcdermott</name><name sortKey="Shrivats, Arun R" sort="Shrivats, Arun R" uniqKey="Shrivats A" first="Arun R" last="Shrivats">Arun R. Shrivats</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Mackenzie, Matthew C" sort="Mackenzie, Matthew C" uniqKey="Mackenzie M" first="Matthew C" last="Mackenzie">Matthew C. Mackenzie</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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